[1]高妍婷,赵文清,石菁,等.萘乙酸诱导玉米瘤黑粉病抗性的生理机制[J].江苏农业学报,2025,(02):242-250.[doi:doi:10.3969/j.issn.1000-4440.2025.02.004]
　GAO Yanting,ZHAO Wenqing,SHI Jing,et al.Physiological mechanism of naphthylacetic acid-induced resistance to common smut in maize[J].,2025,(02):242-250.[doi:doi:10.3969/j.issn.1000-4440.2025.02.004]
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萘乙酸诱导玉米瘤黑粉病抗性的生理机制()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2025年02期
页码:
242-250
栏目:
遗传育种·生理生化
出版日期:
2025-02-28

文章信息/Info

Title:
Physiological mechanism of naphthylacetic acid-induced resistance to common smut in maize
作者:
高妍婷1; 赵文清1; 石菁1; 王芳1; 2; 闫新明1
(1.甘肃农业大学农学院,甘肃兰州730070；2.干旱生境作物学国家重点实验室,甘肃兰州730070)
Author(s):
GAO Yanting1; ZHAO Wenqing1; SHI Jing1; WANG Fang1; 2; YAN Xinming1
(1.College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China;2.State Key Laboratory of Aridland Crop Science, Lanzhou 730070, China)
关键词:
玉米; 萘乙酸; 瘤黑粉病; 抗氧化酶; 防卫基因
Keywords:
maize;  naphthylacetic acid; common smut; antioxidant enzyme; defense gene
分类号:
S435.131.4+1
DOI:
doi:10.3969/j.issn.1000-4440.2025.02.004
文献标志码:
A
摘要:
为明确萘乙酸(NAA)诱导玉米瘤黑粉病抗性的生理机制,本研究以玉米自交系掖478和齐319为材料,分析不同浓度萘乙酸处理对玉米瘤黑粉病病原菌菌丝生长和玉米叶片抗氧化酶活性、抗病相关酶活性、渗透调节物质及玉米相关防卫基因表达量的影响,明确NAA诱导植物抗病性的作用机理。结果表明:2.0 mmol/L NAA对玉米瘤黑粉病病原菌有明显抑制作用,处理玉米叶片后会产生较好的诱抗效果。与单独接种病原菌对照和无菌水对照相比,接种2.0 mmol/L NAA溶液+病原菌后24～48 h的玉米植株抗氧化酶活性、抗病相关酶活性及脯氨酸含量总体呈增加趋势,丙二醛含量明显降低,同时玉米相关防卫基因SOD、PAL、LOX的表达量呈上调趋势。接种后48 h,接种病原菌+2.0 mmol/L NAA 处理的掖478和齐319叶片SOD、PAL、LOX基因相对表达量比单独接种病原菌对照分别增加52.44%和56.83%、43.57%和10.69%、47.44%和81.08%。本研究结果为施用外源NAA防治玉米瘤黑粉病提供了依据。
Abstract:
In order to clarify the physiological mechanism of naphthylacetic acid (NAA)-induced resistance to maize smut, in this study, maize inbred lines Ye478 and Qi319 were used as materials to analyze the effects of different concentrations of NAA on the mycelial growth of Ustilago maydis, the activities of antioxidant enzymes and disease-resistant enzymes, osmotic adjustment substances and the expression of defense-related genes in maize leaves, and to clarify the mechanism of NAA-induced plant disease resistance. The results showed that 2.0 mmol/L NAA had a significant inhibitory effect on the pathogen of maize smut, and it had a good induced resistance effect after treating maize plants. Compared with the control groups inoculated with the pathogen alone and sterile water, the antioxidant enzyme activity, disease resistance-related enzyme activity and proline content of maize plants inoculated with 2.0 mmol/L NAA solution + pathogen for 24-48 h showed an overall increasing trend, and the malondialdehyde content was significantly reduced. At the same time, the expression levels of maize-related defense genes SOD, PAL, and LOX were up-regulated. At 48 h after inoculation, the relative expression levels of SOD, PAL and LOX genes in leaves of Ye478 and Qi319 inocu-lated with pathogen + 2.0 mmol/L NAA increased by 52.44% and 56.83%, 43.57% and 10.69%, 47.44% and 81.08%, respectively, compared with those inoculated with pathogen alone. The results of this study provide a basis for the application of exogenous NAA to control maize smut.

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备注/Memo

备注/Memo:
收稿日期：2024-04-09基金项目：国家自然科学基金地区科学基金项目(32060487)；甘肃省自然科学基金重点项目(23JRRA1405)；大学生创新创业训练计划(SIETP)项目(202301023)作者简介：高妍婷(1999-),女,甘肃景泰人,硕士研究生,主要从事玉米抗病机理研究。(E-mail)3269168831@qq.com通讯作者：王芳,(E-mail)wangfang@gsau.edu.cn
更新日期/Last Update: 2025-03-27