[1]黄正洋,孔令琳,王钱保,等.鸡Fnip1基因克隆、组织表达及生物信息学分析[J].江苏农业学报,2025,(01):119-125.[doi:doi:10.3969/j.issn.1000-4440.2025.01.014]
　HUANG Zhengyang,KONG Linglin,WANG Qianbao,et al.Cloning, tissue expression and bioinformatics analysis of chicken Fnip1 gene[J].,2025,(01):119-125.[doi:doi:10.3969/j.issn.1000-4440.2025.01.014]
点击复制

鸡Fnip1基因克隆、组织表达及生物信息学分析()
分享到：

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2025年01期
页码:
119-125
栏目:
畜牧兽医·水产养殖·益虫饲养
出版日期:
2025-01-31

文章信息/Info

Title:
Cloning, tissue expression and bioinformatics analysis of chicken Fnip1 gene
作者:
黄正洋; 孔令琳; 王钱保; 李春苗; 吴兆林; 黄华云; 赵振华
(江苏省家禽科学研究所,江苏扬州225125)
Author(s):
HUANG Zhengyang; KONG Linglin; WANG Qianbao; LI Chunmiao; WU Zhaolin; HUANG Huayun; ZHAO Zhenhua
(Jiangsu Institute of Poultry Science, Yangzhou 225125, China)
关键词:
鸡; Fnip1; 基因克隆; 表达分析; 生物信息学分析
Keywords:
chicken; Fnip1; gene cloning; expression analysis; bioinformatics analysis
分类号:
S831.2
DOI:
doi:10.3969/j.issn.1000-4440.2025.01.014
文献标志码:
A
摘要:
为了明确鸡卵泡素相互作用蛋白1基因(Fnip1)特征及其表达规律,本研究选择苏禽3号黄羽肉鸡为试验材料,运用分子克隆技术扩增了鸡Fnip1基因CDS区序列全长,对其序列特性进行了生物信息学分析,构建了系统进化树；利用RT-qPCR方法检测了Fnip1基因在鸡不同组织中的表达。结果获得鸡Fnip1基因CDS区,序列开放阅读框为3 474 bp,位于第13号染色体16 191 415 bp至16 251 731 bp之间,编码1 157个氨基酸。生物信息学分析结果显示,Fnip1基因有20个外显子,FNIP1蛋白含有FNIP_N、FNIP_M和FNIP_C等3个结构域；进化树显示,鸡先与鸟类聚为一类,再与哺乳动物聚为一支,在禽类上序列保守。FNIP1蛋白为亲水性蛋白,相对分子量为128 310,理论等电点为5.25。蛋白质二级结构由α-螺旋(34.40%)、β-折叠(4.06%)、延伸链(13.74%)、无规则卷曲(47.80%)组成。表达分析结果显示,Fnip1基因在鸡的胸肌和腿肌中表达量显著高于其他组织。综上所述,本研究获得了鸡Fnip1基因CDS区序列全长,发现其在鸡肌肉组织中有较高表达。本研究结果可为进一步研究Fnip1基因在鸡肌肉生长发育中的分子机制研究奠定数据支撑。
Abstract:
To clarify the characteristics and expression patterns of chicken follistatin-interacting protein 1 gene (Fnip1), Suqin No.3 yellow-feathered broilers were selected as experimental materials in this study, and the full-length sequence of chicken Fnip1 gene CDS region was amplified by molecular cloning technology. Moreover, the bioinformatics analysis of its sequence characteristics was carried out, and the phylogenetic tree was constructed. The expression of Fnip1 gene in different tissues of chickens was detected by RT-qPCR. The results showed that the CDS region of chicken Fnip1 gene was obtained. The open reading frame of the sequence was 3 474 bp, which was located between 16 191 415 bp and 16 251 731 bp on chromosome 13, encoding 1 157 amino acids. Bioinformatics analysis indicated that Fnip1 gene had 20 exons, and FNIP1 protein contained three domains: FNIP_N, FNIP_M and FNIP_C. The phylogenetic tree showed that chickens were first clustered with birds, and then clustered with mammals, and the sequence was conserved in birds. FNIP1 protein was a hydrophilic protein with a size of 128 310 and a theoretical isoelectric point of 5.25. The secondary structure of the protein was composed of α-helix (34.40%), β-sheet (4.06%), extended chains (13.74%) and random coils (47.80%). The expression analysis showed that the expression of Fnip1 gene in chicken breast muscle and leg muscle was significantly higher than that in other tissues. In summary, this study obtained the full-length sequence of the chicken Fnip1 gene CDS region and found that it was highly expressed in chicken muscle tissue. The results of this study can provide data support for further studies on the molecular mechanism of Fnip1 gene in chicken muscle growth and development.

参考文献/References:

[1]SAGER R A, WOODFORD M R, MOLLAPOUR M. The mTOR independent function of Tsc1 and Fnips[J]. Trends Biochemical Sciences,2018,43(12):935-937.
[2]XIAO L, YIN Y, SUN Z, et al. Ampk Phosphorylation of Fnip1(S220)controls mitochondrial function and muscle fuel utilization during exercise[J]. Science Advances,2024,10(6):eadj2752.
[3]YIN Y, XU D, MAO Y, et al. Fnip1 regulates adipocyte browning and systemic glucose homeostasis in mice by shaping intracellular calcium dynamics[J]. Journal of Experimental Medicine,2022,219(5):e20212491.
[4]MALIK N, FERREIRA B I, HOLLSTEIN P E, et al. Induction of lysosomal and mitochondrial biogenesis by AMPK phosphorylation of Fnip1[J]. Science,2023,380(6642):eabj5559.
[5]XIAO L, LIU J, SUN Z, et al. Ampk-dependent and -independent coordination of mitochondrial function and muscle fiber type by Fnip1[J]. PLoS Genetics,2021,17(3):e1009488.
[6]RAMIREZ REYES J M J, CUESTA R, PAUSE A. Folliculin:a regulator of transcription through AMPK and mTOR signaling pathways[J]. Frontiers in Cell and Developmental Biology,2021,9:667311.
[7]MENG J, FERGUSON S M. GATOR1-dependent recruitment of Flcn-Fnip to lysosomes coordinates Rag GTPase heterodimer nucleotide status in response to amino acids[J]. Journal of Cell Biology,2018,217(8):2765-2776.
[8]WOODFORD M R, DUNN D M, BLANDEN A R, et al. The FNIP co-chaperones decelerate the Hsp90 chaperone cycle and enhance drug binding[J]. Nature Communications,2016,7:12037.
[9]ZHOU Q, GU Y, LANG H, et al. Dihydromyricetin prevents obesity-induced slow-twitch-fiber reduction partially via FLCN/FNIP1/AMPK pathway[J]. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease,2017,1863(6):1282-1291.
[10]SCHMIDT L S, LINEHAN W M. Flcn:the causative gene for Birt-Hogg-Dubé syndrome[J]. Gene,2018,640:28-42.
[11]GLYKOFRIDIS I E, KNOL J C, BALK J A, et al. Loss of FLCN-FNIP1/2 induces a non-canonical interferon response in human renal tubular epithelial cells[J]. Elife,2021,10:e61630.
[12]NIEHUES T, ZGR T T, BICKES M, et al. Mutations of the gene Fnip1 associated with a syndromic autosomal recessive immunodeficiency with cardiomyopathy and pre-excitation syndrome[J]. European Journal of Immunology,2020,50(7):1078-1080.
[13]SAGER R A, WOODFORD M R, BACKE S J, et al. Post-translational regulation of FNIP1 creates a rheostat for the molecular chaperone Hsp90[J]. Cell Reports,2019,26(5):1344-1356.
[14]周启程. 二氢杨梅素调控flcn-Fnip1/Ampk通路抑制肥胖诱导的骨骼肌纤维类型转换[D]. 重庆:第三军医大学,2017.
[15]付亮亮. 高低饲料效率猪骨骼肌差异表达蛋白鉴定及肌肉特异表达mir-208b功能研究[D]. 武汉:武汉华中农业大学,2017.
[16]刘静. 骨骼肌能量代谢与肌纤维结构偶联调控机制研究[D]. 南京:南京大学,2017.
[17]MADEIRA F, MADHUSOODANAN N, LEE J, et al. The embl-ebi job dispatcher sequence analysis tools framework in 2024[J]. Nucleic Acids Research,2024,52(1):521-525.
[18]TAMURA K, STECHER G, KUMAR S. MEGA11: molecular evolutionary genetics analysis version 11[J]. Molecular Biology and Evolution,2021,38(7):3022-3027.
[19]SIGGS O M, STOCKENHUBER A, DEOBAGKAR-LELE M, et al. Mutation of Fnip1 is associated with b-cell deficiency,cardiomyopathy,and elevated ampk activity[J]. Proceedings of the National Academy of Sciences of the United States of America,2016,113(26):3706-3715.
[20]LIU J, LIANG X, ZHOU D, et al. Coupling of mitochondrial function and skeletal muscle fiber type by a miR-499/Fnip1/AMPK circuit[J]. EMBO Molecular Medicine,2016,8(10):1212-1228.
[21]BABA M, HONG S B, SHARMA N, et al. Folliculin encoded by the BHD gene interacts with a binding protein,FNIP1,and AMPK,and is involved in AMPK and mTOR signaling[J]. Proceedings of the National Academy of Sciences of the United States of America,2006,103(42):15552-15557.
[22]HASUMI H, BABA M, HASUMI Y, et al. Folliculin-interacting proteins Fnip1 and Fnip2 play critical roles in kidney tumor suppression in cooperation with Flcn[J]. Proceedings of the National Academy of Sciences of the United States of America,2015,112(13):1624-1631.

相似文献/References:

[1]金崇富,杨智青,陈长宽,等.盐城地方鸡MSTN 基因外显子1 多态性及其与生产性能的相关性分析[J].江苏农业学报,2016,(06):1372.[doi:doi:10.3969/j.issn.1000-4440.2016.06.027]
　JIN Chong-fu,YANG Zhi-qing,CHEN Chang-kuan,et al.Association of MSTN gene with growth and reproductive traits in Yancheng chicken[J].,2016,(01):1372.[doi:doi:10.3969/j.issn.1000-4440.2016.06.027]
[2]金崇富,陈长宽,杨智青,等.鸡IGF1R、IGFBP-3基因SNP位点之间互作效应分析[J].江苏农业学报,2015,(05):1099.[doi:doi:10.3969/j.issn.1000-4440.2015.05.024]
　JIN Chong-fu,CHEN Chang-kuan,YANG Zhi-qing,et al.Genetic interaction effect between SNPs loci of IGF1R and IGFBP-3 genes in chicken[J].,2015,(01):1099.[doi:doi:10.3969/j.issn.1000-4440.2015.05.024]
[3]刘青涛,李银,刘娜,等.H9N2亚型禽流感病毒对鸡的免疫抑制机制[J].江苏农业学报,2018,(02):368.[doi:doi:10.3969/j.issn.1000-4440.2018.02.021]
　LIU Qing-tao,LI Yin,LIU Na,et al.The immunosuppressive mechanism of H9N2 subtype avian influenza virus infection in chickens[J].,2018,(01):368.[doi:doi:10.3969/j.issn.1000-4440.2018.02.021]
[4]黄正洋,王钱保,黄华云,等.鸡let-7b在不同生长时期及不同组织中的表达及其生物信息学分析与靶基因预测[J].江苏农业学报,2022,38(02):429.[doi:doi:10.3969/j.issn.1000-4440.2022.02.017]
　HUANG Zheng-yang,WANG Qian-bao,HUANG Hua-yun,et al.Expression of let-7b in different growth stages and different tissues of chicken and its bioinformatics analysis and target gene prediction[J].,2022,38(01):429.[doi:doi:10.3969/j.issn.1000-4440.2022.02.017]
[5]尚书凤,赵小龙,杨书洋,等.1α羟化酶的真核表达和生物信息学特征及其在成年肉鸡组织中的差异表达[J].江苏农业学报,2022,38(05):1286.[doi:doi:10.3969/j.issn.1000-4440.2022.05.016]
　SHANG Shu-feng,ZHAO Xiao-long,YANG Shu-yang,et al.Eukaryotic expression and bioinformatics characteristics of 1α hydroxylase and its differential expression in adult broiler tissues[J].,2022,38(01):1286.[doi:doi:10.3969/j.issn.1000-4440.2022.05.016]
[6]魏清宇,吴鹏飞,叶红心,等.边鸡快长型与慢长型品系胚胎骨骼肌发育中差异可变剪切[J].江苏农业学报,2023,(02):444.[doi:doi:10.3969/j.issn.1000-4440.2023.02.017]
　WEI Qing-yu,WU Peng-fei,YE Hong-xin,et al.Differential alternative splicing during embryonic skeletal muscle development in fast-growing and slow-growing strains of Bian chicken[J].,2023,(01):444.[doi:doi:10.3969/j.issn.1000-4440.2023.02.017]
[7]朱淼,李欣燃.鸡HSP70家族基因鉴定及功能分析[J].江苏农业学报,2023,(03):770.[doi:doi:10.3969/j.issn.1000-4440.2023.03.018]
　ZHU Miao,LI Xin-ran.Identification and functional analysis of the chicken HSP70 family genes[J].,2023,(01):770.[doi:doi:10.3969/j.issn.1000-4440.2023.03.018]

备注/Memo

备注/Memo:
收稿日期：2024-07-10基金项目：现代农业产业技术体系肉鸡体系项目(CARS-41-Z21)；江苏省种业振兴揭榜挂帅项目[JBGS(2021)109)]；扬州市现代农业项目(YZ2023054)作者简介：黄正洋(1987-),男,河南信阳人,博士,副研究员,主要从事家禽遗传育种研究。(E-mail)zyhuang@qq.com。孔令琳为共同第一作者。通讯作者：赵振华,(E-mail)zzh0514@163.com
更新日期/Last Update: 2025-02-28