[1]李明瑾,霍建飞,韩长志,等.辣椒炭疽病病原菌中分泌蛋白的预测及特性分析[J].江苏农业学报,2025,(01):41-50.[doi:doi:10.3969/j.issn.1000-4440.2025.01.006]
　LI Mingjin,HUO Jianfei,HAN Changzhi,et al.Prediction and characteristic analysis of secretory proteins in pepper anthracnose pathogens[J].,2025,(01):41-50.[doi:doi:10.3969/j.issn.1000-4440.2025.01.006]
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辣椒炭疽病病原菌中分泌蛋白的预测及特性分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2025年01期
页码:
41-50
栏目:
植物保护
出版日期:
2025-01-31

文章信息/Info

Title:
Prediction and characteristic analysis of secretory proteins in pepper anthracnose pathogens
作者:
李明瑾1; 2; 霍建飞2; 韩长志1; 姚玉荣2; 贲海燕2; 郝永娟2; 王万立2
(1.西南林业大学林学院/云南省森林灾害预警与控制重点实验室,云南昆明650224；2.天津市农业科学院植物保护研究所,天津300384)
Author(s):
LI Mingjin1; 2; HUO Jianfei2; HAN Changzhi1; YAO Yurong2; BEN Haiyan2; HAO Yongjuan2; WANG Wanli2
(1.Forestry College of Southwest Forestry University/Yunnan Key Laboratory of Forest Disaster Warning and Control, Kunming 650224, China;2.Institute of Plant Protection, Tianjin Academy of Agricultural Sciences, Tianjin 300384, China)
关键词:
辣椒炭疽病病原菌; 分泌蛋白; 生物信息学分析
Keywords:
pepper anthracnose pathogens; secreted proteins; bioinformatics analysis
分类号:
S436.418.1+1
DOI:
doi:10.3969/j.issn.1000-4440.2025.01.006
文献标志码:
A
摘要:
由辣椒炭疽病病原菌引起的辣椒炭疽病严重危害着中国辣椒产业的健康发展。分泌蛋白作为炭疽病病原菌中重要的致病因子,能帮助病原菌侵入寄主组织、分解寄主细胞壁,为病原菌生存和繁殖提供条件。然而,学术界尚缺乏对辣椒炭疽病病原菌中分泌蛋白的深入解析和研究。本研究基于前期获得的辣椒炭疽病病原菌强致病菌株TJNH1全基因组序列,充分利用SignalP、ProtComp等生物信息学分泌蛋白预测软件,对该病菌中13 419条蛋白质氨基酸序列进行预测,同时,基于转录组测序数据,对上述分泌蛋白的功能及特性展开研究。结果表明,辣椒炭疽病病原菌菌株TJNH1中含有388个分泌蛋白,占总蛋白质数量的2.89%,分泌蛋白氨基酸序列长度介于55 aa至730 aa,非极性氨基酸丙氨酸(Ala)在分泌蛋白里使用频率最高,分泌蛋白信号肽长度集中于17 aa至21 aa,信号肽切割位点为A-X-A型。分泌蛋白均为亲水性蛋白,高亲水性氨基酸和高疏水性氨基酸数量最多的分别是天冬酰胺(Asn)和丙氨酸(Ala),分泌蛋白大多为稳定、酸性蛋白。同时运用SMART、eggNOG、STRING V11.5等软件对上述分泌蛋白的保守结构域、COG功能分类、蛋白质互作关系等进行分析,有152个分泌蛋白具有明显的保守结构域,163个分泌蛋白获得了功能注释,32个分泌蛋白存在互作关系。该研究结果为明确分泌蛋白的功能和辣椒炭疽病病原菌的致病机理提供了理论支撑,也为筛选以分泌蛋白为靶标的新型农药和辣椒炭疽病绿色防控提供了理论依据。
Abstract:
Pepper anthracnose caused by the pathogens of pepper anthracnose seriously endangers the healthy development of pepper industry in China. As an important pathogenic factor in anthracnose pathogens, the secretory protein can help pathogens invade host tissues, decompose host cell walls, and provide survival and reproduction conditions for pathogens. However, there is still a lack of in-depth analysis and research on the secreted proteins in the pathogens of pepper anthracnose. In this study, based on the whole genome sequence of the strong pathogenic strain TJNH1 of pepper anthracnose pathogens obtained in the previous study, 13 419 protein amino acid sequences in the pathogen were predicted by using bioinformatics secreted protein prediction software such as SignalP and ProtComp. Meanwhile, based on transcriptome sequencing data, the functions and characteristics of the above-mentioned secreted proteins were studied. The results showed that TJNH1 contained 388 secretory proteins, accounting for 2.89% of the total proteins. The length of amino acid sequences of secreted proteins ranged from 55 aa to 730 aa. The non-polar amino acid alanine (Ala) was the most frequently used in secreted proteins. The length of the secreted protein signal peptide was concentrated from 17 aa to 21 aa, and the signal peptide cleavage site was A-X-A type. The secreted proteins were all hydrophilic proteins. Asparagine (Asn) and alanine (Ala) had the largest number of highly hydrophilic amino acids and highly hydrophobic amino acids, respectively. Most of the secreted proteins were stable and acidic proteins. At the same time, SMART, eggNOG, STRING V11.5 and other software were used to analyze the conserved domains, COG functional classification and protein interaction of the above secreted proteins. There were 152 secreted proteins with obvious conserved domains, 163 secreted proteins were functionally annotated, and 32 secreted proteins had interactions. The results of this study provide a theoretical support for clarifying the functions of secreted proteins and the pathogenic mechanisms of pepper anthracnose pathogens, and also provide a theoretical basis for screening new pesticides targeting secreted proteins and green prevention and control of pepper anthracnose.

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备注/Memo

备注/Memo:
收稿日期：2024-06-03基金项目：天津市农业科学院青年科研人员创新研究与实验项目(2022011)；云南省“兴滇英才支持计划”青年人才专项(YNWR-QNBJ-2020-188)；天津市科技计划项目(23ZYCGSN00220)作者简介：李明瑾(1992-),女,云南昆明人,硕士研究生,主要从事农业资源利用与植物保护研究。(E-mail)1614950297@qq.com通讯作者：霍建飞,(E-mail)hjf2203@163.com；韩长志,(E-mail)swfuhcz@163.com
更新日期/Last Update: 2025-02-28