[1]郭思思,任春梅,李硕,等.玉米黄花叶病毒运动蛋白的特征分析及其在昆虫细胞内的亚细胞定位[J].江苏农业学报,2025,(01):35-40.[doi:doi:10.3969/j.issn.1000-4440.2025.01.005]
　GUO Sisi,REN Chunmei,LI Shuo,et al.Characterization of maize yellow mosaic virus movement protein and its subcellular localization in insect cells[J].,2025,(01):35-40.[doi:doi:10.3969/j.issn.1000-4440.2025.01.005]
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玉米黄花叶病毒运动蛋白的特征分析及其在昆虫细胞内的亚细胞定位()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2025年01期
页码:
35-40
栏目:
植物保护
出版日期:
2025-01-31

文章信息/Info

Title:
Characterization of maize yellow mosaic virus movement protein and its subcellular localization in insect cells
作者:
郭思思1; 2; 任春梅1; 李硕1; 季英华1; 王海涛1; 程兆榜1
(1.江苏省农业科学院植物保护研究所/江苏省食品质量安全重点实验室,江苏南京210014；2.福建农林大学植物保护学院,福建福州350002)
Author(s):
GUO Sisi1; 2; REN Chunmei1; LI Shuo1; JI Yinghua1; WANG Haitao1; CHENG Zhaobang1
(1.Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences/Key Laboratory of Food Quality and Safety of Jiangsu Province, Nanjing 210014, China; 2.College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, China)
关键词:
玉米黄花叶病毒; 运动蛋白; 亚细胞定位
Keywords:
maize yellow mosaic virus; movement protein; subcellular localization
分类号:
S435.13
DOI:
doi:10.3969/j.issn.1000-4440.2025.01.005
文献标志码:
A
摘要:
玉米黄花叶病毒(Maize yellow mosaic virus,MaYMV)是一种具有潜在流行风险的植物病毒,然而目前关于其蛋白质的特征及亚细胞定位情况研究较少。本研究选用2022年采自江苏省的MaYMV作为研究对象,对其运动蛋白(Movement protein, MP)进行分析,结果发现MP为相对分子量21 230的亲水性蛋白质,该蛋白质不含核定位信号、信号肽和跨膜区,二级结构主要呈无规则卷曲形式,且具有Luteo_Vpg功能域。利用Bacmids-MaYMV-MP-His重组穿梭质粒表达系统发现,MP定位于细胞质,进一步通过蛋白质印迹(Western-blot)法验证了MP的表达。该研究探明了MaYMV MP的特征及其亚细胞定位,有望为后续进一步分析MaYMV MP功能及其与介体蚜虫之间的相互作用奠定基础。
Abstract:
Maize yellow mosaic virus (MaYMV) is a plant virus with potential epidemic risks. However, there are few studies on its protein characteristics and subcellular localization. In this study, MaYMV collected from Jiangsu province in 2022 was selected as the research object, and its movement protein (MP) was analyzed. The results showed that MP was a hydrophilic protein with a relative molecular weight of 21 230. The protein did not contain a nuclear localization signal, a signal peptide, or a transmembrane region. Moreover, the secondary structure was mainly in the form of random coils and had a Luteo_Vpg functional domain. Using the Bacmids-MaYMV-MP-His recombinant shuttle plasmid expression system, it was found that MP was localized in the cytoplasm. The expression of MP was further verified by Western-blot. This study demonstrated the characteristics and subcellular localization of MaYMV MP. The results of this study can lay a foundation for further analysis of the function of MaYMV MP and its interaction with vector aphids.

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备注/Memo

备注/Memo:
收稿日期：2024-07-17基金项目：国家自然科学基金项目(32001871)作者简介：郭思思(1999-),女,福建泉州人,硕士研究生,研究方向为植物病理学。(E-mail)547576177@qq.com通讯作者：程兆榜,(E-mail)onlyone8501@126.com；王海涛,(E-mail)wanghtchn1@163.com
更新日期/Last Update: 2025-02-28