[1]常海霞,李静,李明源,等.耐盐产吲哚乙酸(IAA)多功能菌株筛选及对盐胁迫小麦的促生效应[J].江苏农业学报,2025,(01):9-20.[doi:doi:10.3969/j.issn.1000-4440.2025.01.002]
　CHANG Haixia,LI Jing,LI Mingyuan,et al.Screening of multifunctional salt-tolerant indole-3-acetic acid (IAA)-producing strains and their growth-promoting effects on wheat under salt stress[J].,2025,(01):9-20.[doi:doi:10.3969/j.issn.1000-4440.2025.01.002]
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耐盐产吲哚乙酸(IAA)多功能菌株筛选及对盐胁迫小麦的促生效应()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2025年01期
页码:
9-20
栏目:
遗传育种·生理生化
出版日期:
2025-01-31

文章信息/Info

Title:
Screening of multifunctional salt-tolerant indole-3-acetic acid (IAA)-producing strains and their growth-promoting effects on wheat under salt stress
作者:
常海霞; 李静; 李明源; 茹仙古丽·尤努斯; 王继莲
(喀什大学生命与地理科学学院/新疆帕米尔高原生物资源与生态重点实验室,新疆喀什844000)
Author(s):
CHANG Haixia; LI Jing; LI Mingyuan; Ruxiangul·YUNUS; WANG Jilian
(College of Life and Geographic Sciences, Kashi University/Key Laboratory of Biological Resources and Ecology of Pamirs Plateau in Xinjiang Uygur Autonomous Region, Kashi 844000, China)
关键词:
吲哚乙酸; 多功能菌株; 盐胁迫; 促生效应
Keywords:
indole-3-acetic acid; multifunctional strains; salt stress; growth-promoting effect
分类号:
S156.4
DOI:
doi:10.3969/j.issn.1000-4440.2025.01.002
文献标志码:
A
摘要:
吲哚乙酸(Indole-3-acetic acid,IAA)是一种植物激素,对植物种子萌发、根系发育、生长及代谢都有着重要的调控作用。为丰富耐盐碱产IAA的多功能菌种资源,探究菌株的耐盐性及对盐胁迫下植物的促生效果,本研究从新疆克孜勒苏柯尔克孜自治州盐生植物根际、根内、土壤中筛选高产IAA的多功能菌株,从中选取产IAA能力较强的优良菌株接种于盐胁迫下的小麦幼苗验证其促生能力。结果共分离到181株产IAA菌株,其中MHCA37、MPCB16、MHCA17菌株分泌IAA的量位居前3位。将具有IAA分泌能力的菌株进行聚合酶链反应-限制性内切酶片段长度多态性(PCR-RFLP)分析后,选取代表性菌株进行16S rRNA基因测序,结果显示,所有菌株隶属6个菌属,以肠杆菌(Enterobacter)占比最高。产IAA菌株对不同浓度盐胁迫下小麦地上部分和地下部分的影响不同,在150 mmol/L NaCl胁迫下,接种MHCA37、MPCB16和MHCA17菌株的小麦在茎粗、株高、根干重、地上部分干重、叶绿素含量5个方面均显著高于对照；接种MHCA37菌株后小麦幼苗过氧化氢酶(CAT)活性在NaCl浓度为150 mmol/L和200 mmol/L时显著高于对照；接种MHCA17菌株的小麦超氧化物歧化酶(SOD)活性在NaCl浓度为150 mmol/L和200 mmol/L时显著高于对照；接种MHCA37菌株后小麦幼苗过氧化物酶(POD)活性在NaCl浓度为150 mmol/L时显著高于对照,接种MHCA17菌株的小麦幼苗POD活性在NaCl浓度为200 mmol/L时显著高于对照；接种MHCA37、MPCB16、MHCA17菌株的小麦幼苗在NaCl浓度为150 mmol/L轻度盐胁迫下丙二醛（MDA）含量均显著低于对照。据此推测，本研究筛选所得的产IAA菌株在盐胁迫下促进植物生长，提高植物抗逆性等方面有较大应用潜力,同时为开发适用于盐碱地的耐盐多功能微生物菌剂提供了理论依据。
Abstract:
As a plant hormone, indole-3-acetic acid (IAA) plays an important role in plant seed germination, root development, growth and metabolism. To enrich the resources of IAA-producing strains and investigate their growth-promoting effects under salt stress, multifunctional strains with IAA-producing ability were screened from halophytes in saline-alkali land of Kizilsu Kirghiz Autonomous Prefecture, Xinjiang Uygur Autonomous Region. Then the excellent strains were selected and inoculated into wheat seedlings under salt stress to verify their growth-promoting ability. As a result, a total of 181 IAA-producing strains were isolated, among which MHCA37, MPCB16, and MHCA17 were the top three in terms of IAA secretion. The strains with IAA secretion ability were analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), and the representative strains were selected for 16S rRNA gene sequencing. The results showed that all strains belonged to six genera, and Enterobacter was the dominant genus. The effects of IAA-producing strains on the aboveground and underground parts of wheat under different salt stresses were different. Under 150 mmol/L NaCl stress, the stem diameter, plant height, root dry weight, shoot dry weight and chlorophyll content of wheat inoculated with MHCA37, MPCB16 and MHCA17 were significantly higher than those of the control. The catalase (CAT) activity of wheat seedlings inoculated with MHCA37 under 150 mmol/L and 200 mmol/L NaCl treatments was significantly higher than that of the control. The superoxide dismutase (SOD) activity of wheat inoculated with MHCA17 under 150 mmol/L and 200 mmol/L NaCl treatments was significantly higher than that of the control. The peroxidase (POD) activity of wheat seedlings inoculated with MHCA37 strain under 150 mmol/L NaCl treatment was significantly higher than that of the control. The POD activity of wheat seedlings inoculated with MHCA17 strain under 200 mmol/L NaCl treatment was significantly higher than that of the control. The malondialdehyde (MDA) content of wheat seedlings inoculated with MHCA37, MPCB16 and MHCA17 strains under 150 mmol/L NaCl mild salt stress was significantly lower than that of the control. It is speculated that the IAA-producing strains screened in this study have great application potential in promoting plant growth and improving plant stress resistance under salt stress. At the same time, the results of this study provide a theoretical basis for the development of salt-tolerant multifunctional microbial agents suitable for saline-alkali land.

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备注/Memo

备注/Memo:
收稿日期：2024-03-13基金项目：国家自然科学基金项目(32160408)作者简介：常海霞(1999-),女,新疆奎屯人,硕士研究生,主要从事农用微生物资源研究。(E-mail)chang_0513@163.com通讯作者：王继莲,(E-mail)wjilian0710@sina.com
更新日期/Last Update: 2025-02-28