[1]黄静,王文婷,张初署,等.磁性介孔沸石复合材料对花生样品中黄曲霉毒素B1的快速富集[J].江苏农业学报,2024,(11):2177-2186.[doi:doi:10.3969/j.issn.1000-4440.2024.11.021]
　HUANG Jing,WANG Wenting,ZHANG Chushu,et al.Rapid enrichment of magnetic mesoporous zeolite composites on aflatoxin B1 in peanut samples[J].,2024,(11):2177-2186.[doi:doi:10.3969/j.issn.1000-4440.2024.11.021]
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磁性介孔沸石复合材料对花生样品中黄曲霉毒素B1的快速富集()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2024年11期
页码:
2177-2186
栏目:
加工贮藏·质量安全
出版日期:
2024-11-30

文章信息/Info

Title:
Rapid enrichment of magnetic mesoporous zeolite composites on aflatoxin B1 in peanut samples
作者:
黄静1; 王文婷1; 张初署2; 周海翔2; 王冕2; 唐月异2; 朱立飞2; 张建成2
(1.辽宁工程技术大学，辽宁阜新123000；2.山东省花生研究所,山东青岛266000)
Author(s):
HUANG Jing1; WANG Wenting1; ZHANG Chushu2; ZHOU Haixiang2; WANG Mian2; TANG Yueyi2; ZHU Lifei2; ZHANG Jiancheng2
(1.Liaoning Technical University, Fuxin 123000, China;2.Shandong Peanut Research Institute, Qingdao 266000, China)
关键词:
磁性固相萃取; 介孔沸石; 黄曲霉毒素; 花生
Keywords:
magnetic solid phase extraction; mesoporous zeolite; aflatoxin; peanut
分类号:
TQ424
DOI:
doi:10.3969/j.issn.1000-4440.2024.11.021
文献标志码:
A
摘要:
本研究通过改性沸石介孔性能的方法制备新型吸附材料磁性介孔沸石,利用磁性分散固相萃取结合高效液相色谱法,建立了花生中黄曲霉毒素B1 (AFB1)的提取方法。通过场发射扫描电子显微镜、傅立叶变换红外光谱、比表面积和磁性分析技术分析材料理化性质。结果显示，磁性介孔沸石复合材料Fe3O4/zeolite-b杂质更少、孔洞更多、内径更大且孔道结构更加明显,并且孔径大小集中、平均孔径较大,具有超强磁性；将磁性介孔沸石材料作为吸附剂,通过优化萃取条件,结合高效液相色谱-荧光检测器检测花生样品中的AFB1。结果表明,当磁性介孔沸石的添加量为25 mg,pH为7,样品体积为1.0 mL,吸附时间为1 min,洗脱剂为乙腈,洗脱剂体积为3 mL,洗脱时间为3 min时,AFB1质量浓度为2.00～20.00 μg/L,目标物色谱峰面积与质量浓度有良好的线性关系,决定系数（R2）为0.976 8,方法检测限0.05 μg/L,定量限0.16 μg/L。3种不同加标质量浓度(2.00 μg/L、10.00 μg/L、20.00 μg/L)下,AFB1加标回收率范围为81.3%～92.6%,相对标准偏差为2.2%～3.4%。
Abstract:
In this study, a new adsorption material magnetic mesoporous zeolite was prepared by modifying the mesoporous properties of zeolite. The extraction method of aflatoxin B1 (AFB1) from peanut was established by magnetic dispersion solid phase extraction combined with high performance liquid chromatography. The physical and chemical properties of the materials were analyzed by field emission scanning electron microscopy (FE-SEM), Fourier transform infrared spectroscopy (FT-IR), Brunauer-Emmett-Teller (BET) and hysteresis loop (VSM). The results showed that Fe3O4/zeolite-b composite materials had less impurities, more holes, larger inner diameter and more obvious pore structure, and the pore size was concentrated, the average pore size was larger, which had super magnetic properties. The magnetic mesoporous zeolite material was used as adsorbent to detect AFB1 in peanut samples by optimizing extraction conditions and combining with high performance liquid chromatography-fluorescence detector (FD). The results showed that the mass concentration of AFB1 was 2.00-20.00 μg/L when the addition amount of magnetic mesoporous zeolite was 25 mg, the pH was seven, the sample volume was 1.0 mL, the extraction time was 1 min, the elution agent was acetonitrile, the elution volume was 3 mL, the adsorption time was 3 min, and the chromatographic peak area of the target object had a good linear relationship with the mass concentration. The coefficient of determination (R2) was 0.976 8, the detection limit was 0.05 μg/L, and the quantitation limit was 0.16 μg/L. Under three different mass concentrations (2.00 μg/L, 10.00 μg/L, 20.00 μg/L), the recoveries of AFB1 were 81.3%-92.6%, and the relative standard deviations were 2.2%-3.4%.

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备注/Memo

备注/Memo:
收稿日期：2024-10-24基金项目：山东省农业科学院农业科技创新工程项目(CXGC2023A39)；山东省重点研发计划(乡村振兴科技创新)项目(2022TZXD0031)；山东省自然科学基金面上项目(ZR2020MC103、ZR2021MC040)作者简介：黄静(1978-),女,辽宁沈阳人,博士,副教授,主要从事生物化学及复合材料的研究。(Tel)15841835211；(E-mail)15918893@qq.com通讯作者：张建成,(Tel)13863920622；(E-mail)jianc
更新日期/Last Update: 2025-01-20