[1]王占军,李静静,周子寒,等.油桐ARF基因家族的鉴定及表达分析[J].江苏农业学报,2024,(06):984-992.[doi:doi:10.3969/j.issn.1000-4440.2024.06.004]
　WANG Zhanjun,LI Jingjing,ZHOU Zihan,et al.Identification and expression analysis of ARF gene family in Vernicia fordii[J].,2024,(06):984-992.[doi:doi:10.3969/j.issn.1000-4440.2024.06.004]
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油桐ARF基因家族的鉴定及表达分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2024年06期
页码:
984-992
栏目:
遗传育种·生理生化
出版日期:
2024-06-30

文章信息/Info

Title:
Identification and expression analysis of ARF gene family in Vernicia fordii
作者:
王占军1; 2; 李静静1; 周子寒1; 孙婉1; 王毅1; 张勰2
(1.合肥师范学院生物与食品工程学院,安徽合肥230601；2.湖南省林业科学院省部共建木本油料资源利用国家重点实验室,湖南长沙410004)
Author(s):
WANG Zhanjun1; 2; LI Jingjing1; ZHOU Zihan1; SUN Wan1; WANG Yi1; ZHANG Xie2
(1.School of Biology and Food Engineering, Hefei Normal University, Hefei 230601, China；2.State Key Laboratory of Utilization of Woody Oil Resource, Hunan Academy of Forestry, Changsha 410004, China)
关键词:
油桐; ARF基因家族; 生物信息学分析; 表达分析
Keywords:
Vernicia fordii; ARF gene family; bioinformatics analysis; expression analysis
分类号:
S794.3
DOI:
doi:10.3969/j.issn.1000-4440.2024.06.004
摘要:
植物生长发育由多种激素共同调控,其中生长素是重要的调控激素之一。生长素响应因子(Auxin response factor,ARF)是生长素信号传导过程中的关键因子,它能够特异性地与生长素应答元件结合,进而影响植物体内生长素响应基因的表达。本研究通过生物信息学方法鉴定到17个油桐ARF基因家族成员,分布在9条染色体上,并且各基因间无片段重复现象。理化性质分析结果表明,ARF蛋白长度为587～1 119 aa,均为酸性蛋白质,不稳定系数＞40,为不稳定蛋白质,亲水性指数＜0,为亲水性蛋白质。根据系统进化分析结果将油桐ARF蛋白家族分为4个亚族,与拟南芥的亚族划分一致。每个成员都具有典型的B3型结构域和Auxin_resp中间结构域。分析ARF基因表达特征发现,ARF基因的表达在油桐不同组织中具有明显的组织特异性。本研究结果为揭示油桐生长发育调控机制提供了理论依据。
Abstract:
Plant growth and development is regulated by many hormones, among which auxin is one of the important regulatory hormones. Auxin response factor (ARF) is a key factor in the process of auxin signal transduction, which can specifically bind to auxin response elements and affect the expression of auxin response genes in plants. In this study, 17 members of ARF gene family in Vernicia fordii were identified by bioinformatics method, which were distributed on nine chromosomes, and there was no fragment duplication between genes. The results of physical and chemical properties analysis showed that the length of ARF proteins was 587-1 119 aa, all of them were acid proteins. The instability coefficients of ARF proteins were more than 40, and the ARF proteins were unstable proteins. The hydropathicity indexes of ARF proteins were less than 0, and the ARF proteins were hydrophilic proteins. According to the results of phylogenetic analysis, the ARF protein family of Vernicia fordii was divided into four subfamilies, which was consistent with the subfamily division of Arabidopsis thaliana. Each member had a typical B3 domain and an auxin_resp intermediate domain. By analyzing the expression characteristics of ARF gene, it was found that the expression of ARF gene had obvious tissue specificity in different tissues of Vernicia fordii. The results of this study provide a theoretical basis for revealing the regulatory mechanism of Vernicia fordii growth and development.

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相似文献/References:

[1]王占军,汪虹妍,杨妍萍,等.油桐HSP70基因家族的全基因组鉴定与表达分析[J].江苏农业学报,2024,(05):806.[doi:doi:10.3969/j.issn.1000-4440.2024.05.005]
　WANG Zhanjun,WANG Hongyan,YANG Yanping,et al.Genome-wide identification and expression analysis of HSP70 gene family in Vernicia fordii[J].,2024,(06):806.[doi:doi:10.3969/j.issn.1000-4440.2024.05.005]

备注/Memo

备注/Memo:
收稿日期：2024-02-05基金项目：省部共建木本油料资源利用国家重点实验室开放课题(GZKF202103、GZKF202203)；安徽省高校优秀青年人才支持计划项目(gxyq2020040)；安徽省级大学生创新创业训练计划项目(S202414098204、S202414098205)；国家级大学生创新创业训练计划项目(202414098077);2022年度引进高层次人才科研启动基金项目（2022rcjj12）作者简介：王占军(1984-),男,安徽寿县人,博士研究生,从事植物细胞分子生物学研究。(E-mail)wangzhanjunhxj@163.com。李静静为共同第一作者。通讯作者：张勰,(E-mail)zhangxiei3@163.com
更新日期/Last Update: 2024-07-15