[1]张明媛,冷淼,孙伟娜,等.SNP分子标记结合表型性状鉴别小豆种质资源[J].江苏农业学报,2024,(06):975-983.[doi:doi:10.3969/j.issn.1000-4440.2024.06.003]
　ZHANG Mingyuan,LENG Miao,SUN Weina,et al.Identification of adzuki bean (Vigna angularis) germplasm resources based on SNP molecular markers and phenotypic traits[J].,2024,(06):975-983.[doi:doi:10.3969/j.issn.1000-4440.2024.06.003]
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SNP分子标记结合表型性状鉴别小豆种质资源()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2024年06期
页码:
975-983
栏目:
遗传育种·生理生化
出版日期:
2024-06-30

文章信息/Info

Title:
Identification of adzuki bean (Vigna angularis) germplasm resources based on SNP molecular markers and phenotypic traits
作者:
张明媛; 冷淼; 孙伟娜; 柯希望; 徐晓丹; 左豫虎
(黑龙江省作物有害生物互作生物学及生态防控重点实验室/国家杂粮工程技术研究中心/黑龙江八一农垦大学,黑龙江大庆163319)
Author(s):
ZHANG Mingyuan; LENG Miao; SUN Weina; KE Xiwang; XU Xiaodan; ZUO Yuhu
(Heilongjiang Provincial Key Laboratory of Crop-Pest Interaction Biology and Ecological Control/National Coarse Cereals Engineering Research Center/Heilongjiang Bayi Agricultural University, Daqing 163319, China)
关键词:
小豆; 指纹图谱; 质量性状; 种质资源鉴定
Keywords:
adzuki bean; fingerprint; qualitative traits; germplasm resource identification
分类号:
S521
DOI:
doi:10.3969/j.issn.1000-4440.2024.06.003
摘要:
为建立科学可靠的小豆种质资源鉴定方法,本研究利用单核苷酸多态性（SNP）分子标记,对102份小豆种质资源进行聚类分析并构建指纹图谱,同时结合质量性状对小豆种质资源进行鉴定。结果发现,在102份小豆种质资源中26个SNP分子标记表现出多态性,平均多态信息含量为0.28。61份小豆种质资源的SNP指纹图谱具有唯一性,可以被准确鉴别,剩余41份小豆种质资源的SNP指纹图谱不唯一。进一步对102份小豆种质资源的6个质量性状进行遗传变异分析,平均遗传多样性指数为1.224 5,6个性状均具有丰富的多态性。结合以上6个性状,可对SNP指纹图谱相同的种质资源进行进一步区分。本研究开发的SNP分子标记结合表型性状的鉴别方法,可为小豆种质资源遗传鉴定和科学利用奠定基础。
Abstract:
In order to establish a scientific and reliable method for identification of adzuki bean [Vigna angularis (Willd) Ohwi ＆ H. Ohashi] germplasm resources, 102 adzuki bean germplasm resources were clustered and fingerprinted by single nucleotide polymorphism (SNP) molecular markers, at the same time, the germplasm resources of adzuki bean were identified combined with qualitative traits. The results showed that 26 SNP molecular markers in 102 adzuki bean germplasm resources showed polymorphism, and the average polymorphism information content was 0.28. The SNP fingerprints of 61 adzuki bean germplasm resources were unique and could be accurately identified. The SNP fingerprints of the remaining 41 adzuki bean germplasm resources were not unique. The average genetic diversity index of six qualitative traits was 1.224 5 in 102 germplasm resources through further analysis of genetic variation, and all of the six traits were highly polymorphic. Combined with the above six traits, germplasm resources with the same SNP fingerprints can be further distinguished. The identification method of SNP molecular markers combined with phenotypic traits developed in this study can lay a foundation for genetic identification and scientific utilization of adzuki bean germplasm resources.

参考文献/References:

[1]林汝法,柴岩,廖琴. 中国小杂粮[M]. 北京:中国农业技术科学出版社,2002:210-227.
[2]王丽侠,王素华,常汝镇,等. 小豆种质资源研究与利用概述[J]. 植物遗传资源学报,2013,14(3):440-447.
[3]颜军,杨旭红,王雨,等. 我国小豆品种选育与新品种保护进展[J]. 种子,2021,40(4):51-58.
[4]段永红,渠云芳. 遗传标记在植物研究中的应用[M]. 北京:中国农业科学技术出版社,2010:4.
[5]白鹏,程须珍,王丽侠,等. 小豆种质资源农艺性状综合鉴定与评价[J]. 植物遗传资源学报,2014,15(6):1209-1215.
[6]何伟锋,叶福民,乔辉,等. 辽宁省小豆种质资源表型鉴定及多样性分析[J]. 河北农业大学学报,2022,45(1):20-29.
[7]贾继增. 分子标记种质资源鉴定和分子标记育种[J]. 中国农业科学,1996,29(4):1-10.
[8]BUTTON P. The international union for the protection of new varieties of plants (UPOV) recommendations on variety denominations[J]. Acta Horticulturae,2008,799:191-200.
[9]赵波,金文林,濮绍京,等. 基于SSR小豆新品种(系)DNA指纹图谱构建及亲缘分类初探[J]. 北京农学院学报,2008,23(3):1-6.
[10]颜军,焦雄飞,张凯淅,等. 基于SSR标记的小豆品种鉴定体系建立及应用[J]. 分子植物育种,2023,21(2):525-543.
[11]刘洪,徐振江,饶得花,等. 基于形态学性状和SSR标记的花生品种遗传多样性分析和特异性鉴定[J]. 作物学报,2019,45(1):26-36.
[12]徐晓丹,冷淼,张明媛,等. 基于转录组的小豆SSR分子标记开发及其应用[J]. 干旱地区农业研究,2023,41(1):13-18.
[13]HELYAR S J, HEMMER H J, BEKKEVOLD D, et al. Application of SNPs for population genetics of nonmodel organisms:new opportunities and challenges[J]. Molecular Ecology Resources,2011,11(1):123-136.
[14]LI Y, YANG K, YANG W, et al. Identification of QTL and qualitative trait loci for agronomic traits using SNP markers in the adzuki bean[J]. Frontiers in Plant Science,2017,8(8):840.
[15]MASAHIKO M, KENTO M, TSUBASA K, et al. Mapping of QTLs controlling epicotyl length in adzuki bean (Vigna angularis)[J]. Breeding Science,2021,71(2):208-216.
[16]袁明灯,江国彬,王瑞江. 国产耳草属长节耳草及其相似种的分类研究[J]. 热带亚热带植物学报,2022,30(3):377-391.
[17]魏朔南,赵喜萍,田敏爵,等. 应用植物形态学和AFLP分子标记鉴别陕西漆树品种[J]. 西北植物学报,2010,30(4):665-671.
[18]SAGHAI M A, SOLIMAN K M, JORGENSEN R A, et al. Ribosomal DNA spacer-length polymorphisms in barley:mendelian inheritance,chromosomal location,and population dynamics[J]. Proceedings of the National Academy of Sciences,1984,81(24):8014-8018.
[19]BOTSTEIN D R, WHITE R L, SKOLNICK M H, et al. Construction of a genetic linkage map in man using restriction fragment length polymorphisms[J]. The American Journal of Human Genetics,1980,32(3):314-331.
[20]刘欣,程瑞,徐兵划,等. 基于KASP技术的SNP标记用于西瓜品种指纹图谱构建和种子纯度检测[J]. 江苏农业学报,2022,38(5):1348-1356.
[21]程须珍,王素华,王丽侠. 小豆种质资源描述规范和数据标准[M]. 北京:中国农业出版社,2006:12-75.
[22]张昆鹏. 利用SNP标记构建油菜品种指纹图谱及定位下卷叶性状基因的研究[D]. 南京:南京农业大学,2013.
[23]王泽涵,于文涛,樊晓静,等. 利用SNP标记构建漳州南部茶树种质资源的分子身份证[J]. 江苏农业科学,2022,50(18):284-289.
[24]颜廷进,蒲艳艳,张文兰,等. 基于SNP标记的菜豆品种真实性和纯度鉴定技术[J]. 山东农业科学,2019,51(12):111-119.
[25]JOS A C, JAVIER I, DIEGO L, et al. A 48 SNP set for grapevine cultivar identification[J]. BMC Plant Biology,2011,11(1):153.
[26]JING J K, PARK S W, LIU W Y, et al. Discovery of single nucleotide polymorphism in Capsicum and SNP markers for cultivar identification[J]. Euphytica,2010,175(1):91-107.
[27]魏中艳,李慧慧,李骏,等. 应用SNP精准鉴定大豆种质及构建可扫描身份证[J]. 作物学报,2018,44(3):315-323.
[28]HOREJSI T, STAUB J E. Genetic variation in cucumber (Cucumis sativus L.) as assessed by random amplified polymorphic DNA[J]. Genetic Resources and Crop Evolution,1999,46(4):337-350.
[29]龙珏臣,杜成章,张晓春,等. 重庆小豆种质资源的遗传多样性分析及白粉病抗性鉴定[J]. 四川农业大学学报,2022,40(4):481-488.
[30]金文林,陈迎春,陈丽燕,等. 小豆杂交后代主要农艺性状的遗传参数分析[J]. 北京农学院学报,1997(2):1-9.
[31]AOYAMA S, ONISHI K, KATO K. The genetically unstable dwarf locus in azuki bean [Vigna angularis (Willd) Ohwi & Ohashi][J]. J Hered,2011,102(5):604-609.
[32]吕建珍,王宏勇,任莹,等. 不同生态区谷子品种表型鉴定及SSR遗传多样性分析[J]. 核农学报,2023,37(3):471-482.

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备注/Memo

备注/Memo:
收稿日期：2023-07-05基金项目：黑龙江八一农垦大学校启动计划项目(2031011068)；大庆市科学技术局指导项目(zd-2020-45)作者简介：张明媛(1996-),女,河北邢台人,硕士研究生,主要从事植物病理学研究。(E-mail)zhangmingyuan2333@gmail.com通讯作者：徐晓丹,(E-mail)xxdalice@163.com；左豫虎,(E-mail)zuoyuhu@byau.edu.cn
更新日期/Last Update: 2024-07-15